Non-Shine-Dalgarno Translation Initiation and Regulatory Mechanisms

Nunez, Nathaniel, Nadra Alhusini, and Jared Schrader

It is generally accepted that prokaryotic translation is governed and initiated through base paring of the Shine-Dalgarno sequence on mRNA with the anti-Shine-Dalgarno sequence on the 16s ribosomal subunit. Despite this foundational translation model proposed through work in E. coli, recent studies have shown that a myriad of bacterial species, including many clinically important species such as Mycoplasma genitalium utilize Shine Dalgarno mediated translation in as little as 8 percent of their genome but these organisms have slow doubling times and lack genetic tools. Caulobacter crescentus is a fast growing, genetically tractable, non-pathogenic organism with Shine-Dalgarno sites in <24% of its genes making it an ideal model organism to study non-Shine-Dalgarno translation. This research aims to quantify the role of initiation factors and ribosomal proteins on non-Shine-Dalgarno translation in C. crescentus. These initiation factors and ribosomal proteins will be purified for an in vitro and in vivo analysis of translational efficiency on non-Shine-Dalgarno mRNAs.