Understanding the Role of Tumor Suppressor Protein Cables 1 in Cell Cycle Regulation

Polack, Meghan, Nolan Patel, Kirby Swan, Khanh Nguyen, and Stephanie Conant

The goal of our study is to understand tumor growth in humans by studying a knockdown of a cell cycle regulator and tumor suppressor protein named Cables 1.  Mutations in the Cables 1 gene have been observed in human colon, lung, endometrial, and ovarian tissue tumors.  The downstream effect of a loss of functional Cables 1 protein and tumor suppression that may lead to the development of cancer has yet to be defined.  This lab uses a small-interfering RNA (siRNA) approach to disrupt the transcription and translation of the Cables 1 protein in a mammalian cell-line and mimic naturally occurring mutations in this tumor suppressor protein. This siRNA approach will allow us to identify loss of the Cables 1 protein and determine downstream influence of Cables 1 on other cell cycle regulator proteins that may be involved in preventing the development of tissue-specific tumors.  Understanding how disruption of the production of Cables 1 drives tumor growth could help to identify novel molecular markers that can be utilized in diagnosis and therapeutic treatment of cancer.